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blue-fluorescent dna stain 4',6-diamidino-2-phenylindole dapi  (Thermo Fisher)


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    Thermo Fisher blue-fluorescent dna stain 4',6-diamidino-2-phenylindole dapi
    Blue Fluorescent Dna Stain 4',6 Diamidino 2 Phenylindole Dapi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescent+dna+stain+dapi/blue+fluorescent+dna+stain+4++6+diamidino+2+phenylindole+dapi/us12070504-599-7-17
    Average 90 stars, based on 1 article reviews
    blue-fluorescent dna stain 4',6-diamidino-2-phenylindole dapi - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Staining:

    Article Title: EMBRYONIC DEVELOPMENT AND A QUANTITATIVE MODEL OF PROGRAMMED DNA ELIMINATION IN MESOCYCLOPS EDAX (S. A. FORBES, 1891) (COPEPODA: CYCLOPOIDA)
    Article Snippet: .. Histological Stains and Specimen Preparation The fluorescent DNA stain DAPI (4′-6-diamidino-2-phenylindole; Thermo Fisher Scientific, Waltham, MA, USA) was used to label the pronuclei prior to their fusion. ..

    Article Title: Embryonic development and a quantitative model of programmed DNA elimination in Mesocyclops edax (S. A. Forbes, 1891) (Copepoda: Cyclopoida)
    Article Snippet: .. Histological Stains and Specimen Preparation The fluorescent DNA stain DAPI (4′-6-diamidino-2-phenylindole; Thermo Fisher Scientific, Waltham, MA, USA) was used to label the pronuclei prior to their fusion. ..

    Article Title: Two glycosyl transferase 2 genes from the gram-positive bacterium Clostridium ventriculi encode (1,3;1,4)-β-D-glucan synthases.
    Article Snippet: They were then harvested by centrifugation (4000 ×g, 3 min) and incubated with the primary antibody, a mouse monoclonal IgG antibody against (1,3;1,4)-β-D-glucan (BS400–3) (Biosupplies Australia Pty Ltd) diluted 1:100 with 0.1 % BSA in PBS, and incubated at 4 ◦C overnight. .. The spheroplasts were then washed by centrifugation (4000 ×g, 3 min, 4 ◦C) in PBS (3 times) and resuspended in the secondary antibody, goat anti-mouse IgG (H + L) conjugated to the fluorophore AlexaFluorTM 555 (Invitrogen, Waltham, MA, US), diluted 1:100 with 0.1 (w/v) BSA in PBS and incubated in the dark at RT for 1 h. They were then washed in the same way as after the incubation with the primary antibody, aliquots pipetted onto microscope slides, and stained with the fluorescent DNA stain DAPI in SlowFadeTM antifade mountant (Thermo Fisher) (10 μL). .. After incubating in the dark for at least min, the slides were examined with an inverted confocal microscope (Leica Stellaris 8) using a 100× oil objective lens.

    Article Title: EMBRYONIC DEVELOPMENT AND A QUANTITATIVE MODEL OF PROGRAMMED DNA ELIMINATION IN MESOCYCLOPS EDAX (S. A. FORBES, 1891) (COPEPODA: CYCLOPOIDA)
    Article Snippet: .. The fluorescent DNA stain DAPI (4′-6-diamidino-2-phenylindole; Thermo Fisher Scientific, Waltham, MA, USA) was used to label the pronuclei prior to their fusion. ..

    Article Title: The Shiga Toxin Receptor Globotriaosylceramide as Therapeutic Target in Shiga Toxin E. coli Mediated HUS
    Article Snippet: Mouse anti-CD31 (BD Biosciences, San Jose, CA, USA) primary and corresponding species-specific Alexa Fluor 568 were used as antibodies. .. Alexa 488-labeled Stx-B at a concentration of 5 μg/mL and the fluorescent DNA stain DAPI (Invitrogen, Carlsbad, CA, USA) were added, and images were taken with the use of a Zeiss LSM900 confocal microscope (Zeiss, Oberkochen, Baden-Württemberg, Germany). ..

    Article Title: Development of experimental in vitro burn model.
    Article Snippet: .. Nuclei were counter-stained with blue fluorescent DNA stain DAPI (1:1000), Molecular Probe life technologies, for 10 min at 22°C. .. The images are a composite of 3 images acquired using filter sets appropriate for blue, green and red fluorescence, on a Confocal Laser Scanning Microscope Leica - TCS SP8 (Germany) as adapted from Gaiba et al. (2012).

    Article Title: Cell Division in Staphylococcus Aureus : Protein-Protein Interactions and Super-Resolution Microscopy
    Article Snippet: .. For this purpose, the fluorescent DNA stain DAPI (4’6-diamidino-2-phenylindole [Molecular Probes]) and the lipophilic probe FM 4-64 (Invitrogen) was used to label S. aureus DNA and cell membranes, respectively. ..

    Centrifugation:

    Article Title: Two glycosyl transferase 2 genes from the gram-positive bacterium Clostridium ventriculi encode (1,3;1,4)-β-D-glucan synthases.
    Article Snippet: They were then harvested by centrifugation (4000 ×g, 3 min) and incubated with the primary antibody, a mouse monoclonal IgG antibody against (1,3;1,4)-β-D-glucan (BS400–3) (Biosupplies Australia Pty Ltd) diluted 1:100 with 0.1 % BSA in PBS, and incubated at 4 ◦C overnight. .. The spheroplasts were then washed by centrifugation (4000 ×g, 3 min, 4 ◦C) in PBS (3 times) and resuspended in the secondary antibody, goat anti-mouse IgG (H + L) conjugated to the fluorophore AlexaFluorTM 555 (Invitrogen, Waltham, MA, US), diluted 1:100 with 0.1 (w/v) BSA in PBS and incubated in the dark at RT for 1 h. They were then washed in the same way as after the incubation with the primary antibody, aliquots pipetted onto microscope slides, and stained with the fluorescent DNA stain DAPI in SlowFadeTM antifade mountant (Thermo Fisher) (10 μL). .. After incubating in the dark for at least min, the slides were examined with an inverted confocal microscope (Leica Stellaris 8) using a 100× oil objective lens.

    Incubation:

    Article Title: Two glycosyl transferase 2 genes from the gram-positive bacterium Clostridium ventriculi encode (1,3;1,4)-β-D-glucan synthases.
    Article Snippet: They were then harvested by centrifugation (4000 ×g, 3 min) and incubated with the primary antibody, a mouse monoclonal IgG antibody against (1,3;1,4)-β-D-glucan (BS400–3) (Biosupplies Australia Pty Ltd) diluted 1:100 with 0.1 % BSA in PBS, and incubated at 4 ◦C overnight. .. The spheroplasts were then washed by centrifugation (4000 ×g, 3 min, 4 ◦C) in PBS (3 times) and resuspended in the secondary antibody, goat anti-mouse IgG (H + L) conjugated to the fluorophore AlexaFluorTM 555 (Invitrogen, Waltham, MA, US), diluted 1:100 with 0.1 (w/v) BSA in PBS and incubated in the dark at RT for 1 h. They were then washed in the same way as after the incubation with the primary antibody, aliquots pipetted onto microscope slides, and stained with the fluorescent DNA stain DAPI in SlowFadeTM antifade mountant (Thermo Fisher) (10 μL). .. After incubating in the dark for at least min, the slides were examined with an inverted confocal microscope (Leica Stellaris 8) using a 100× oil objective lens.

    Microscopy:

    Article Title: Two glycosyl transferase 2 genes from the gram-positive bacterium Clostridium ventriculi encode (1,3;1,4)-β-D-glucan synthases.
    Article Snippet: They were then harvested by centrifugation (4000 ×g, 3 min) and incubated with the primary antibody, a mouse monoclonal IgG antibody against (1,3;1,4)-β-D-glucan (BS400–3) (Biosupplies Australia Pty Ltd) diluted 1:100 with 0.1 % BSA in PBS, and incubated at 4 ◦C overnight. .. The spheroplasts were then washed by centrifugation (4000 ×g, 3 min, 4 ◦C) in PBS (3 times) and resuspended in the secondary antibody, goat anti-mouse IgG (H + L) conjugated to the fluorophore AlexaFluorTM 555 (Invitrogen, Waltham, MA, US), diluted 1:100 with 0.1 (w/v) BSA in PBS and incubated in the dark at RT for 1 h. They were then washed in the same way as after the incubation with the primary antibody, aliquots pipetted onto microscope slides, and stained with the fluorescent DNA stain DAPI in SlowFadeTM antifade mountant (Thermo Fisher) (10 μL). .. After incubating in the dark for at least min, the slides were examined with an inverted confocal microscope (Leica Stellaris 8) using a 100× oil objective lens.

    Article Title: The Shiga Toxin Receptor Globotriaosylceramide as Therapeutic Target in Shiga Toxin E. coli Mediated HUS
    Article Snippet: Mouse anti-CD31 (BD Biosciences, San Jose, CA, USA) primary and corresponding species-specific Alexa Fluor 568 were used as antibodies. .. Alexa 488-labeled Stx-B at a concentration of 5 μg/mL and the fluorescent DNA stain DAPI (Invitrogen, Carlsbad, CA, USA) were added, and images were taken with the use of a Zeiss LSM900 confocal microscope (Zeiss, Oberkochen, Baden-Württemberg, Germany). ..

    other:

    Article Title: Identification of a longevity gene through evolutionary rate covariation of insect mito-nuclear genomes.
    Article Snippet: O xi da tive p ho sphorylation, essential for energy metabolism and linked to the regulation of longevity, involves mitochondrial and nuclear genes.. The functions of these genes and their evolutionary rate covariation (ERC) have been extensively studied, but little is known about whether other nuclear genes not targeted to mitochondria evolutionarily and functionally interact with mitochondrial genes.. Here we systematically examined the ERC of mitochondrial and nuclear benchmarking universal single-copy ortholog (BUSCO) genes from 472 insects, identifying 75 non-mitochondria-targeted nuclear genes.

    Concentration Assay:

    Article Title: The Shiga Toxin Receptor Globotriaosylceramide as Therapeutic Target in Shiga Toxin E. coli Mediated HUS
    Article Snippet: Mouse anti-CD31 (BD Biosciences, San Jose, CA, USA) primary and corresponding species-specific Alexa Fluor 568 were used as antibodies. .. Alexa 488-labeled Stx-B at a concentration of 5 μg/mL and the fluorescent DNA stain DAPI (Invitrogen, Carlsbad, CA, USA) were added, and images were taken with the use of a Zeiss LSM900 confocal microscope (Zeiss, Oberkochen, Baden-Württemberg, Germany). ..



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